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    Graphene ,Magnetism, the jab and the Human Brain Project

    THEeXchanger
    THEeXchanger
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    • Post n°101

    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Empty Re: Graphene ,Magnetism, the jab and the Human Brain Project

    Post  THEeXchanger Wed Sep 01, 2021 1:16 pm

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    THEeXchanger
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    • Post n°102

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    Post  THEeXchanger Wed Sep 01, 2021 1:28 pm

    PATENT PENDING 
    - does protect the item / corporation or person who manufactured the item
    because all the paperwork required for a patent has been completed, filed and submitted to the patent office 
    and, thus, it can be advertised and manufactured, and, imprinted with the words 'patent pending' on it 
    and, that item can enjoy the 'full protection' of an item that has a 'patent number'...
    the earliest date of filing, is the most important thing related to a patent, as, to who will end up owning it,
    if 2 or more of a similar item, files around the same time for a patent !!!
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    THEeXchanger
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    • Post n°103

    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Empty Re: Graphene ,Magnetism, the jab and the Human Brain Project

    Post  THEeXchanger Thu Sep 02, 2021 11:21 pm

    [ltr]2 SEPARATE IMPERATIVE SCIENTIFIC FACT & ISSUES
    1.] CORONAVIRUS (99% SURVIVABLE), this topic is finished/closed, irrelevant
    2.] S-PROTEINS ("SPIKE PROTEINS") GRAPHENE, just starting, BIGGER THAN COV-19

    BOTTOM LINE?
    Graphene is in the vaccine, as is mRNA, the vaccinated are spreading S-Proteins ("spike proteins") through natural behavior, thus the unvaccinated are indirectly vaccinated, as a result, our DNA (deoxyribonucleic acid, the "blueprint of life," gold standard) is altered and we will undoubtedly be physically affected.

    How does the mRNA COVID-19 vaccine work?
    mRNA vaccines teach our cells how to make a protein—or even just a piece of a protein—that triggers an immune response inside our bodies.

    How does the Johnson & Johnson COVID-19 vaccine differ from mRNA?
    The ultimate difference is the way the instructions are delivered. The Moderna and Pfizer vaccines use mRNA technology, and the Johnson & Johnson vaccine uses the more traditional virus-based technology. mRNA is essentially a little piece of code that the vaccine delivers to your cells

    WHO INVENTED GRAPHENE? Physic Professors Andre Geim, Kostya Novoselov, 2004

    GRAPHENE STRENGTH : it's the lightest, strongest, thinnest, best heat, electricity conducting material ever discovered, stronger than diamonds, stops bullets, stronger than Kevlar because the flat planes of carbon atoms in graphene flexes without atoms breaking.

    IS GRAPHENE HARMFUL TO HUMANS? Yes, the jagged edges of graphene nanoparticles are super sharp, strong, easily pierces cell membranes in the human lung, skin, immune cells, suggesting serious damage in humans and animals.

    SOURCES :

    [size=0]https://www.    bitchute.com/video/ejxBXze9S5uq/

    https://www.    graphene-info.com/graphene-introduction

    https://www.graphene-info.com/graphene-dna-sequencing[/ltr][/size]
    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 313f63b24190080d

    GRAPHENE 666 : The Untold Story (Russ Brown Reports)
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    Vidya Moksha
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    • Post n°104

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    Post  Vidya Moksha Sun Sep 12, 2021 6:06 am

    Curious.. !

    why are all the words graphene highlighted in red in this thread? Shocked

    or at least that's how they are showing on my screen Suspect

    anyway...

    I have given up on this idea of graphene in the kill shot. I think its mis-direction.

    I stumbled across this today.
    https://www.reuters.com/article/factcheck-graphene-lipidvaccines-idUSL1N2PI2XH

    Fact Check-No evidence graphene oxide is present in available COVID-19 vaccines via lipid nanoparticles

    while i normally take fact checkers as they are intended (a bankster tool) I did think this may be true.
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    Vidya Moksha
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    • Post n°105

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    Post  Vidya Moksha Sun Sep 12, 2021 6:08 am

    I wasnt imagining it.. I looked back through the thread and all the words graphene were in red. Now they are black again.. I didnt do a search or anything, just opened up the last pages
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    Carol
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    • Post n°106

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    Post  Carol Sun Sep 12, 2021 9:09 am

    If you were doing a search for Graphene then all the words would show up in red. Then when you were back in the link they would appear normal.

    Here's some more info on Graphene and Pine Needle tea. Fortunately we're surrounded by pine trees so we're not likely to run out. We like how it smells and tastes. We have a variety of pine trees but stick with the white pine.

    PINE NEEDLE TEA

    Pine needles, Spruce, Cedar and Fir (conifers), contain Shikimate (Shikimic Acid), and a slew of other meta nutrients which boost immunity, hydrate, and contribute to the detoxification of Graphene Oxide Nanoparticles at a cellular level.

    Conifer needle teas inhibit adverse reactions from graphene oxide and Covid-19 spike protein transmission and protect against components of the coagulation cascade; possibly protecting against blood clots. Pine tea also inhibits the inappropriate replication and modification of RNA and DNA.

    Conifer needle oil and needle tea rejuvenates cells and act as a natural stress reliever, pain killer, and antibiotic. It treats every kind of pain, stress, trauma, and PTSD because it works directly on the nerves, bypassing your nervous system. It’s one of the few meta nutrients which erases cellular memory of trauma. Essentially, everyone should be drinking pine needle tea or taking pine oil.

    Order pine needles here. https://metanutrients.com


    _________________
    What is life?
    It is the flash of a firefly in the night, the breath of a buffalo in the wintertime. It is the little shadow which runs across the grass and loses itself in the sunset.
    With deepest respect ~ Aloha & Mahalo, Carol
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    mudra
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    • Post n°107

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    Post  mudra Fri Sep 24, 2021 12:59 am

    beaconterraone says: 09/23/2021 at 10:49 am

    Until a competent lab obtains a solid chain-of-custody-traceable vial of each “product” and then does a full analysis via GC/MS and microscopy, we just don’t know for certain what is actually in these vials. Unfortunately, it also appears, from scientific fact above “conspiracy theory” (sic – LOL), that there are multiple versions of each “product,” with one vial having more “special ingredients” than others. Do they contain graphene? Probably. But that hasn’t been ascertained yet. The La Quinta Columna “analysis” was a joke. wrote:
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    mudra
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    • Post n°108

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    Post  mudra Tue Sep 28, 2021 5:46 pm


    N-Acetylcysteine

    N-Acetylcysteine (NAC) is a prodrug for L-cysteine, which is used for the intention of allowing more glutathione to be produced when it would normally be depleted. Through glutathione buffering, NAC provides antioxidative effects and other benefits.


    https://examine.com/supplements/n-acetylcysteine/?fbclid=IwAR04j23vzIERckFAGOqKbJa2rG7aQFtHXOLhP2BwBp38BrIxCGTmMSPnzmE
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    Carol
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    • Post n°109

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    Post  Carol Thu Sep 30, 2021 7:44 am

    [size=30]Graphene Oxide Detox Protocols For The Vaxxed & Unvaxxed[/size]

     Sat 1:59 pm +00:00, 28 Aug 2021  
    https://tapnewswire.com/2021/08/graphene-oxide-detox-protocols-for-the-vaxxed-unvaxxed/

    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Graphene-Oxide-Detox-Protocols-For-The-Vaxxed-Unvaxxed-FI-08-26-21-min-300x135

    [size=30]Graphene Oxide Detox Protocols For The Vaxxed & Unvaxxed[/size]


    By Dr. Ariyana Love, ND

    Updated August 25th 2021
    There are many people now experiencing jabbers remorse and want to know what they can do to detoxify Graphene Oxide Nanoparticles and the gain-of-function spike protein they were injected with. Meanwhile, the unvaxxed are experiencing Adverse Events and magnetism due to transmission.
    This article contains all the known safe and effective detox protocols that both the vaxxed and the unvaxxed can use to help your body remove these deadly poisons.
    DR. ZELENKO’S PROTOCOL
    Dr. Vladimir (Zev) Zelenko is a Board Certified Family Physician. He was the first in America (March 2020) to innovate a successful treatment for covid-19.
    A Nobel Prize nominee, Dr. Zelenko was censored and de-platformed across big tech for sharing his life-saving formula and for affirming that Covid-19 is a “bioweapon for mass Genocide”.
    Dr. Zelenko’s Protocol contains Ivermectin, Hydroxychloroquine (HCQ), Zinc, Vitamin D3, and Quercetin. See Dr. Zelenko’s Protocol here. Read more about Dr. Zelenko’s Protocol and watch his latest very important interview, here.
    Order from Dr. Zelenko’s products, here.
    America’s Frontline Doctors are helping people obtain Ivermectin, here.
    Hospitals can purchase Ivermectin for critical care, here.
    Scientific studies:
    Over 100 studies proving that Vitamin D3 is essential for treating Covid-19 can be viewed, here.
    A Slovakia research team discovered under microscopy that Ivermectin halts the crystalline growth of Graphene Oxide Hydrogels inside your body, here.
    VITAMIN C
    This study demonstrates that Ascorbic Acid (Vitamin C) is very effective at reducing Graphene Oxide Toxicity from the body, here. And this study shows that high dose Vitamin C is also an effective treatment for Covid-19, even for the critically ill, here. Finally, intravenous Vitamin C can be successfully used to treat patients with Covid-19, here.
    Important note:
    If you take more than 10,000 international units of Vitamin D3 per day, you must stop consuming all dairy and Vitamin C supplements in order to avoid calcium clots.
    N-ACETYLCLSTEINE (NAC)
    The research of La Quinta Columna led by Ricardo Delgado, successfully tested an inexpensive way to remove magnetic Graphene Oxide Nanoparticles from the human body after they were injected with a Covid jab using N-acetyl-cysteine (NAC) and Zinc.
    “These two antioxidants are essential to degrade Graphene Oxide,” says Delgardo. NAC causes your body to secrete glutathione endogenously and glutathione can reduce Graphene Oxide Toxicity down to zero. In this article and video, you will see Delgado describe what to do, here.
    Scientific studies:
    A study published in PubMed reveals that biocompatible NAC reduces Graphene Oxide, here. In an animal study where enhanced spike protein was used to cause lung damage to animals by binding to the ACE2 receptors, the animals were remedied using NAC, here. Read more about NAC here.
    Instructions:
    La Quinta Columna recommends taking NAC 600-750mg, first thing in the morning on an empty stomach. Also take 2x tablets of Zinc, 25mg each.
    Secondary: Astaxanthin 5mg, Querectin, milk thistle, Vitamin D3.
    DR. RIMA’S PROTOCOL
    Dr. Rima Laibow recommends taking 900mg of NAC per day. Some people are even taking 1400mg. Since NAC is an amino acid, it’s safe to consume it in a non-pharmaceutical, natural form. If using a synthetic pharmaceutical NAC, please consult a physician on dosage.
    In addition to NAC, Dr. Rima also uses the following protocol.
    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Photo_2021-08-23_23-28-12Dr. Rima’s Protocol
    You can purchase Dr. Rima products here.
    While NAC is effective at enabling your cells to produce glutathione, it has some limitations. For example, it’s more effective when injected vs ingested orally. Also, its effectiveness starts decreasing after about 3 months so a long-term solution must be used.
    ASEA REDOX SIGNALING MOLECULES
    It has been demonstrated that Graphene Oxide Toxicity causes intracellular oxidative stress, leading to cytotoxicity and the inhibition of cell proliferation. Glutathione is one of the main body antioxidants that eradicates free radicals and poisons from your body. Glutathione is a cell-signaling molecule created by our cells and used by our bodies at the speed of light. Glutathione is vital in the regulation of oxidative stress levels to maintain normal cellular function. However, its concentration decreases with age, and people are already deficient in glutathione.
    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 3ec88218532157e5bb380aca50fbfccb
    ASEA Redox signaling molecules is called the ‘Water of Life’ because it reactivates gene signalling pathways which get shut off by Graphene Oxide Toxicity. ASEA Redox enables your cells to increase the endogenous glutathione production by a whopping 500-800%, thus detoxifying Graphene Oxide Nanoparticles and spike protein, optimally.
    According to scientific research, ASEA Redox signaling molecules increase the capacity of cells to detox by 4 fold and may increase mitochondria production by 30% after a fortnight. This meta anti-oxidant has the potency of an antidote and because it’s native to the body, the benefits of consuming ASEA will increase with continued use. These anti-aging cell signaling molecules are also good for teeth and gums.
    ASEA was originally discovered and developed by a Biotech firm. An atomic medical physicist specialized in nanotechnology and a Nobel Prize winner figured out how to stabilize the molecules for human consumption. There are years of research and a plethora of doctors behind ASEA. While the pharmaceutical industry attempted to suppress this medical breakthrough, ASEA’s founders had an integral mission to make their product widely available for public use.
    ASEA has the power to potentially save the lives of the vaxxed and will detox transmission in the unvaxxed. It’s potentially the strongest single treatment that’s mentioned in this article.
    You can become an ASEA distributor for as little as $40 US Dollars. I welcome anyone who wishes to spread this information far and wide. Contact me for details, metanutrients@mailfence.com.
    ORDER Asea’s Redox Signaling molecules, here.
    Scientific research:
    This initial gene study showed ASEA Redox affected important signaling pathway genes, [url=https://mediafilelibrary.myasealive.com/src/media/xmfl/file/ASEA REDOX Gene Science Summary EN.pdf]here[/url].
    SURAMIN
    It’s worth mentioning that whistleblower Dr. Judy Mikovitz went on record stating that Suramin is the ‘antidote’ to the Covid-19 bioweapons, here. Suramin is a pharmaceutical drug that is derived from pine needles. Dr. Mikovitz states that a small amount of Suramin injected into the body is sufficient. Consult a doctor before using.
    PINE NEEDLE TEA
    Pine needles, Spruce, Cedar and Fir (conifers), contain Shikimate (Shikimic Acid), and a slew of other meta nutrients which boost immunity, hydrate, and contribute to the detoxification of Graphene Oxide Nanoparticles at a cellular level.
    Conifer needle teas inhibit adverse reactions from graphene oxide and Covid-19 spike protein transmission and protect against components of the coagulation cascade; possibly protecting against blood clots. Pine tea also inhibits the inappropriate replication and modification of RNA and DNA.
    Conifer needle oil and needle tea rejuvenates cells and act as a natural stress reliever, pain killer, and antibiotic. It treats every kind of pain, stress, trauma, and PTSD because it works directly on the nerves, bypassing your nervous system. It’s one of the few meta nutrients which erases cellular memory of trauma. Essentially, everyone should be drinking pine needle tea or taking pine oil.
    Order pine needles here.
    SHIKIMATE
    Shikimate has been used in traditional Chinese medicine to halt plagues, and pandemics. Shikimate halts respiratory infections and viral replication. It can be found in high doses in pine needle tea. It’s also found in a large quantity in Star Anise, Fennel, and dandelion root, leaf and flower.
    World renown Scientist Mike Adams the Health Ranger and founder of Natural News, explains how you can easily extract Shikimate from these herbs using an espresso machine, here. Like other experts, the Health Ranger expresses his belief that the vaxxed may still be able to save themselves, here.
    DAVID WOLF’S PROTOCOL
    It’s very important to consume as many foods, herbs, spices, and natural medicines as you can which contain Shikimate. David Avocado Wolf is a world-renown health guru. Please follow David Avocado Wolf’s Protocol and learn how to order the world’s finest natural products in “Summary of the Spike Protein Protocol”, here.
    Important note:
    Please only consume natural forms of C60 offered by David Avocado Wolf. C60 in its pharmaceutical form must be avoided due to overtly toxic properties. Similarly, iodine found in bleached table salt is a poison and must be avoided. Your body simply will not assimilate it.
    Natural forms of iodine can be assimilated and absorbed by your body, like the iodine found in vegetables and herbs.
    Order from David Wolf’s Shop, here.
    HYDRATION
    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Photo_2021-08-25_15-51-22ACEA distributor Dr. Ariyana Love, ND
    Email: metanutrients@protonmail.com
    Hydration is key to your health, to detoxification, and to using these protocols: all of them. If your body is dehydrated it cannot properly absorb the nutrition particles from what you consume and that nutrition will be flushed out and lost.
    Right now, 97% of the world’s population is dehydrated and 76% is chronically dehydrated. This is due to the majority of our drinking water is acidic based and the molecules are simply too big to be absorbed by our cells.
    Dehydration hinders cell communication. Drinking 8 glasses of water per day will not hydrate you unless it contains electrolytes.
    It’s extremely important to keep your body’s PH level in balance. You want to be always in an alkaline state. An Acidic body is a breeding ground for disease. Electrolytes will not only keep you hydrated but will also balance PH.
    Pine needle tea is a natural electrolyte and very hydrating. Sea salt is also a natural electrolyte. Normal sea salt has between 16-24 minerals but Himalayan salt has 87 minerals which is the exact same saline as your blood. So toss out your table salt and replace it with Himalayan salt.
    Hydration formula:
    Add about a quarter of a teaspoon of Himalayan salt to one liter of water. Squeeze a quarter of fresh lime or lemon juice (lime is less astringent and just as good) into your glass of Himalayan charged water each time you drink. This is a complete electrolyte formula that will fully hydrate you.
    ANTIOXIDANTS AND ROOIBOS TEA
    Consume foods, herbs, and medicines that are high in antioxidants which enable your body to detoxify poisons. African bush tea called Rooibos is a meta antioxidant and is widely available in supermarkets, worldwide.
    Asians are believed to have the lowest cancer rates because of their daily consumption of green tea. Drinking one cup of Rooibos tea is equivalent to consuming 50 cups of green tea in its antioxidant effect. Rooibos is also very hydrating.
    ADDITIONAL INFORMATION
    Dr. Zelenko explains that “the Covid-19 poison death shots create killer antibodies and killer antibodies are time bombs that get triggered by exposure to matching viral infections”. NANA-ME may stop that from happening. N-Acetyl Neuraminic Acid Methyl Ester (NANA-ME).
    Dr. Zelenko further says that “NANE-ME may prevent Antibody-Dependent Enhancement (ADE) and potentially billions of deaths”. Please see the study for treating adverse reactions caused by pathological antibodies induced by Covid-19 and vaccines here.
    Investigative Journalist Ramola D. has additional recommendations for detoxifying Graphene Oxide (GO) Nanoparticles after exposure from chemtrails, here.
    Dr. Carrie Madej suggests regular detox baths with Bentonite Clay to help your body remove GO Nanoparticles.
    CBD oil and Cannabis will help your body cleanse GO Nanoparticles.
    Chlorine Oxide can also detox GO.
    Sodium bicarbonate E500 (baking soda) will help to keep your PH level in an alkaline balance.
    Super Blue Green Algae is a miraculous meta nutrient which chelates the blood and removes heavy metals, here.
    Saladmaster cookware enables you to retain up to 98% of nutrition when cooking your food. Saladmaster pans are made from titanium and surgical stainless steel and do not leach any metals into your food.
    To minimize transmission, I suggest showering after being around the general population. Either wash your clothes immediately or keep them in a plastic bag until you wash them.
    I will be adding updates to this article periodically.

    ••••

    See original article here

    ••••

    Read more COVID related articles by Ariyana

    ••••

    https://www.thelibertybeacon.com/graphene-oxide-detox-protocols-for-the-vaxxed-unvaxxed/


    _________________
    What is life?
    It is the flash of a firefly in the night, the breath of a buffalo in the wintertime. It is the little shadow which runs across the grass and loses itself in the sunset.
    With deepest respect ~ Aloha & Mahalo, Carol
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    Carol
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    • Post n°110

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    Post  Carol Thu Sep 30, 2021 7:46 am

    A drug candidate for treating adverse reactions caused by pathogenic antibodies inducible by COVID-19 virus and vaccines


    https://www.biorxiv.org/content/10.1101/2021.07.13.452194v2.full.pdf


    Huiru Wang1* , Xiancong Wu2 , Yuekai Zhang3 , Qiuchi Chen 2 , Lin Dai2 , Yuxing Chen2 , and Xiaoling Liu2* 1. Huirui Biopharma. Hangzhou, China 2. HuaAn McAb Biotechnology. Hangzhou, China 3. Biolynx Technology. Hangzhou, China Summary In a recent study, we reported that certain anti-spike antibodies of COVID-19 and SARS-CoV viruses can have a pathogenic effect through binding to sick lung epithelium cells and misleading immune responses to attack self-cells. We termed this new pathogenic mechanism “Antibody Dependent Auto-Attack” (ADAA). This study explores a drug candidate for prevention and treatment of such ADAA-based diseases. The drug candidate is a formulation comprising N-acetylneuraminic acid methyl ester (NANA-Me), an analog of N-acetylneuraminic acid. NANA-Me acts through a unique mechanism of action (MOA) which is repairment of the missing sialic acid on sick lung epithelium cells. This MOA can block the antibodies’ binding to sick cells, which are vulnerable to pathogenic antibodies. Our in vivo data showed that the formulation significantly reduced the sickness and deaths caused by pathogenic anti-spike antibodies. Therefore, the formulation has the potential to prevent and treat the serious conditions caused by pathogenic antibodies during a COVID-19 infection. In addition, the formulation has potential to prevent and treat the adverse reactions of COVID-19 vaccines because the vaccines can induce similar antibodies, including pathogenic antibodies. The formulation will be helpful in increasing the safety of the vaccines without reducing the vaccine’s efficacy. Compared to existing antiviral drugs, the formulation has a unique MOA of targeting receptors, broad spectrum of indications, excellent safety profile, resistance to mutations, and can be easily produced. Keywords: COVID-19 infection, drug candidate, pathogenic antibodies, SARS-CoV-2 virus, SARS-CoV virus, new mechanism of action (MOA), adverse reactions of vaccines (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.13.452194; this version posted August 18, 2021. The copyright holder for this preprint 2 and lead to serious clinical conditions including acute respiratory distress syndrome (ARDS), cytokine storms, and death6 . We have termed this novel mechanism “Antibody Dependent Auto-Attack” (ADAA) in a recent article. Since anti-viral antibodies continue to exist for a period of time in recovered COVID-19 patients, certain pathogenic antibodies among them can induce the symptoms of long haulers6 . Given the similarities of antibodies inducible by COVID-19 vaccines, the pathogenic anti-spike antibodies, despite only being minority of the antibodies induced, provided a possible cause for the adverse reactions of COVID-19 vaccines. Therefore, a treatment capable of blocking the binding of pathogenic antibodies to host cells will be effective for the ADAA-based diseases, including adverse reactions of vaccines. Sialic acids are rich on the outer surface of cell membranes and mainly act as biological masks 8 . Cells or tissues with sialic acid are recognized as “self”. After the loss of sialic acids the cellular structures become “non-self” 8 , which can activate immune responses. In a recent report, we have shown that damaged lung epithelium cells with missing sialic acid and human inflammatory tissues are particularly vulnerable to pathogenic anti-spike antibodies6 . During the COVID-19 infection, the sialic acid on lung epithelium cells can be removed by the receptor-destroying enzyme (RDE) of SARS-CoV-2 virus9 , that makes the damaged cells vulnerable to pathogenic antibodies. Thus, repairment of the missing sialic acid can convert the sick cells (non-self) back to normal (self) and block the binding of pathogenic antibodies to those vulnerable cells. This newly discovered mechanism of action (MOA) provides a possible new direction for developing anti-viral drugs. Therefore, we designed a formulation consisting of an analog of sialic acid for repairing the missed sialic acid on the vulnerable cells. In this study, we tested the formulation’s efficacy in preventing and treating adverse reactions caused by pathogenic anti-spike antibodies of COVID-19 and SARS-CoV viruses. Results The composition of a formulation A successuful drug for repairment of missing sialic acid on the cell surface should have three attributes: 1) easily enter cells; 2) participate in the sugar chain synthesis process without affecting the glycosidic linkages; and 3) be stable in vivo. Sialic acid has a negative charge on its surface and cannot easily enter cells and participate in the sugar chain synthesis process, and is metabolized rapidly in vivo 8 . Therefore it is difficult for sialic acid alone to be used as a therapeutic medicine. mg/ml mg/ml A：NANA B: NANA-Me C: NANA (A)+NANA-Me (B) D: NANA-Me repairment B u f e rA :B -1 :5A :B -1 :2 A :B -1 :1 .2 5A :B -1 :1 1 5 0 0 0 2 0 0 0 0 2 5 0 0 0 N A N A /N A N A -M e M FI U n tre a te d S ia lid a s e c u t S ia lid a s e + B H -1 0 3 5 0 0 0 0 0 1 0 0 0 0 0 0 1 5 0 0 0 0 0 W G A v s A 5 4 9 c e lls M F I M e an U n tre a ted S ia lid a s e c u t S ia lid a s e + B H -1 0 3 Figure 1. The sialic acid levels on the surface of human lung epithelium A549 cells determined by a flow cytometry analysis. A549 cells were treated with A: N-acetylneuraminic acid (NANA); B: N-acetylneu- raminic acid methyl ester (NANA-Me); and C: NANA+ NANA-Me. D: BH-103 treated A549 cells with missing sialic acid on the cell surface (sialidase treated), compared to control cells of healthy (intact) or damaged A549 cells (sialidase treated) alone. (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.13.452194; this version posted August 18, 2021. The copyright holder for this preprint 3 N-acetylneuraminic acid methyl ester (NANA-Me) is an analog of N-acetylneuraminic acid (NANA) with a structure close to the structure of NANA. It is easier for NANA-Me to enter cells compared with NANA. However, NANA-Me is unstable at about pH 7.0 (unpublished data). Our study found that the NANA-Me was more stable under the pH conditions of 4.0-5.5 (unpublished data). We further found that the stability of NANA-Me was better when it was in a composition comprising NANA under certain ratio range (NANA-Me:NANA, 1-5:1) of the two compounds (unpublished data, patent pending). Therefore, we designed a formulation consisting of NANA-Me and NANA with a ratio of NANA-Me:NANA at 2:1 (pH 4.43). The formulation was named as BH-103 (patent pending). Repairment of cell surface sialic acid by NANA-Me The sialic acid repairment function of NANA-Me was tested by a cellular assay. Lung epithelium cell line A549 was cultured with NANA-Me or NANA with various concentrations for overnight, and the sialic acid levels on the cells’ surfaces were determined next day with fluorescent labeled-wheat germ agglutinin (WGA) (Vector) and flow cytometry as described in methods. The sialic acid levels of the A549 cells treated with NANA-Me increased in a way of dose dependent between the range of 1mg/ml - 50 mg/ml, while the sialic acid levels of the A549 cells treated with NANA were not changed significantly (FIG 1A and 1B). The data suggests that NANA-Me is helpful in the synthesis and expression of the sialic acid on lung epithelium cells. In another test, the A549 cells were cultured with 50 mg/ml of NANA-Me in combination with NANA at various ratios (NANA-Me:NANA) for overnight. The sialic acid levels on the cells were determined next day with the same method as described above. The results showed that the sialic acid levels on the A549 cells treated with the compositions comprising NANA-Me and NANA at the ratios of 1.25:1 or 2:1 (NANA-Me:NANA) were higher compared with the buffer (vehicle) treated control cells (FIG 1C). Thus, a formulation consisting of NANA-Me and NANA at a ratio of 2:1 (pH 4.43 when dissolved), was prepared and named as BH-103. In order to induce damaged cells, the A549 cells were treated with neuraminidase or sialidase (Roche, Shanghai) according to manufacturer’s instructions 6 . The sialidase treated A549 cells were cultured without or with 50 mg/ml of BH-103 (NANA-Me) at 37⁰C for overnight, and the sialic acid level on A549 cells were determined the next day. As shown in FIG 3D, the sialic acid levels of the sialidase treated A549 cells were N B 4 + W G A B H -1 0 3 + W G A 2 0 0 0 0 0 3 0 0 0 0 0 4 0 0 0 0 0 5 0 0 0 0 0 W G A v s N B 4 c e lls M F I M e an N B 4 + W G A B H -1 0 3 + W G A 12% A N B 4 + S -R B D B H -1 0 3 + S -R B D 1 0 0 0 2 0 0 0 3 0 0 0 4 0 0 0 S -R B D v s N B 4 c e lls M F I M e an N B 4 +S -R B D B H -1 0 3 +S -R B D 26% B C u t+ W G A C u t+ B H -1 0 3 + W G A 0 1 0 2 0 3 0 W G A v s D a m a g e d N B 4 c e lls P o s itiv e c e lls %( ) 31% C S -R B D C u t+ B H -1 0 3 + S -R B D 0 2 0 4 0 6 0 8 0 S -R B D v s N B 4 c e lls P o s itiv e c e lls (% ) S -R B D C u t+ B H -1 0 3 + S -R B D 89% D Figure 2. The levels of sialic acid or S-RBD of SARS-CoV-2 virus on the surface of acute promyelocytic leukemia NB4 cells, determined by a flow cytometry analysis. A: NB4 cells were treated with or without BH-103; B: Binding of S-RBD to NB4 cells with or without BH-103 treatment; C: Damaged NB4 cells (sialidase treated) were treated with or without BH-103; and D: Binding of S-RBD to damaged NB4 cells with or without BH-103 treatment. (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.13.452194; this version posted August 18, 2021. The copyright holder for this preprint 4 lower than that of the untreated cells, indicating loss of sialic acid on A549 cells after being digested with sialidase. The sialic acid levels of the A549 cells treated with sialidase and BH-103 was higher than that of control cells treated with sialidase but without BH-103. Taken together, the data of the in vitro analysis indicated that N-acetylneuraminic acid methyl ester has the potential to enhance the expression of sialic acid and to repair the missed sialic acid on the A549 cell surface. The best enhancing or repairing effect of NANA-Me could be achieved in combination with NANA (pH 4.43). The repairment of sialic acid on cell surfaces is helpful for recovery of damaged (e.g. infected or inflamed) cells, blocking the self-attacking response of the immune system and reducing the severity of diseases and preventing deaths. Blocking the entry of COVID-19 virus The angiotensin converting enzyme 2 (ACE2) is an entry receptor for coronaviruses such as SARS-CoV-2. N-acetylneuraminic acid is a component of ACE which is responsible for the attachment of a coronavirus to the receptor on host cells 9 . We then asked a question: could expression of more sialic acid on cell surfaces increase the receptor amount and infectibility of viruses? To answer the question, we developed an in vitro assay for the viral entry study of the SARS-CoV-2 virus. The human acute promyelocytic leukemia cell line, NB4 cells express ACE2 (The Human Protein Atlas) and was used for the assay. The receptor binding domain (RBD) of the spike protein (S-RBD) of the SARS-CoV-2 virus is responsible for viral entry. The recombinant protein of S-RBD of the SARS-CoV-2 virus (with human Fc as a tag for detection) was purchased (Sino Biological, Beijing) and used for the viral entry assay. In one test, NB4 cells were cultured with BH-103 as described above, at 50 mg/ml of NANA-Me for overnight, and the sialic acid levels on the cells were determined next day as described above. The results showed that the sialic acid levels on the NB4 cells treated with BH-103 were higher compared with untreated control cells (FIG 2A), indicating the enhancing effect of BH-103 on the sialic acid expression of the NB4 cells. In a parallel test the NB4 cells, with and without treatment of BH-103, were incubated with the recombinant S-RBD of the COVID-19 virus in ice for one hour and then stained with a fluorescent (PE) labeled anti-human antibody, followed by a flow cytometry analysis. The results showed that the S-RBD bound on the NB4 cells treated with BH-103 was lower compared with that of the control cells without BH-103 treatment (FIG 4B). In another test, NB4 cells were treated with neuraminidase, then the sialidase treated NB4 cells were cultured without or with 50 mg/ml of BH-103 for overnight. Next day, the sialic acid levels on the NB4 cells were determined. In a parallel test the binding of the recombinant S-RBD to the sialidase treated NB4 cells with or without treatment of BH-103 were tested as described above. As shown in FIG 2C, the sialic acid levels of the sialidase treated NB4 cells was lower than that of untreated cells, indicating loss of sialic acid on NB4 cells after being digested with sialidase. The sialic acid levels of the NB4 cells treated with sialidase and BH-103 was higher than that of control cells treated with sialidase alone. Nevertheless, 89% of the binding of the COVID-19 S-RBD to the NB4 cells treated with sialidase and BH-103 was reduced compared to the control cells treated with sialidase alone (FIG 2D). This result indicated that replacement of NANA by NANA-Me induced a structural change or chemical modification of the viral receptor. The data demonstrated that BH-103 not only repaired the missed sialic acid but also significantly decreased the binding affinity of the COVID-19 S-RBD simultaneously. Taken together, the data indicated that the replacement of NANA by NANA-Me could induce a structural or chemical modification of the viral receptor that significantly decreases the binding affinity of the COVID-19 virus. Therefore, despite the sialic acid expression on the BH-103 treated NB4 cells being higher, the bound S-RBD was significantly lower, especially with the BH-103-treated damaged cells with missing sialic acid. This is because the majority NANA was replaced by NANA-Me on the BH-103-treated damaged cells. The prevention and treatment of the adverse reactions of pathogenic antibodies with BH-103 Our recent study has reported a timed-pregnant disease mouse model induced by pathogenic anti-spike antibodies of SARS-CoV-2 and the SARS-CoV viruses6 . (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.13.452194; this version posted August 18, 2021. The copyright holder for this preprint 5 The mouse model was used in the current study to evaluate the therapeutic effect of BH-103 for the prevention and treatment of the disorders caused by the pathogenic anti-coronavirus antibodies. Like our previous study, the four pathogenic anti-spike antibodies, the polyclonal rabbit anti-spike (S1) of SARS-CoV-2 (antiCOVID-19 S1), anti-spike glycoprotein (S) of SARS-CoV (anti-SARS S), human monoclonal anti-S1 antibodies of SARS-CoV-2, REGN10987 and B38, were used to create the mouse disease model. The control antibodies included purified IgG of sera from healthy rabbit and human, as well as the monoclonal anti-S1 antibody, CR3022-b6. Two dosages of each purified and endotoxin-free antibody IgG were injected intraperitoneally (IP) into timed-pregnant mice of C57BL/6J twice every three days at pregnancy (embryonic) days E15 and E18, respectively. In addition, injection (IP) of BH-103 (15 mg/kg) at either day before or the same day of each pathogenic antibody injection were administered. The frequencies of sickness and death of the fetus and newborn mouse pups are summarized in Table 1. Injection of the four pathogenic antibodies of anti-COVID-19 S1, anti-SARS S, REGN10987 and B38 into pregnant mice induced significant fetal and neonatal death of the mouse pups born to those dames (Table 1). However, the treatment of BH-103 significantly decreased the rates of sickness and death caused by the four pathogenic antibodies (Table 1). Histology changes. The tissue sections of lungs, brains, hearts, kidneys, intestines, and livers from the newborn mouse pups were stained with hematoxylin-eosin (HE) 0 10 20 30 40 50 60 70 Table 1. The sick and death rates of mouse pups born to the dames with the injection of anti-coronavirus antibodies Injected IgG of N = Sick (%) Death (%) Sick+Dead (%) Odds Ratio 95% CI P value None (blank) 7 0 14.3 14.3 NA NA NA Vehicle (saline) 9 11.1 0.00 11.1 0.75 0.04-14.6 1.00 Healthy rabbit serum 6 0 16.7 16.7 1.2 0.06 - 25 1.00 Healthy human serum 10 0 10.0 10 0.67 0.03 - 13 1.00 Anti-COVID-19 S1 22 28.6 28.6 57.1 9.60 1.02 - 90 0.04 Anti-COVID-19 S1+BH-103 11 9.09 0.00 9.09 0.08 0.01 - 0.77 0.02 Anti-SARS S 8 50.0 12.5 62.5 13.3 1.07 - 166 0.05 Anti-SARS S+BH-103 16 6.25 6.25 12.5 0.09 0.01 - 0.67 0.02 MAb-CR3022-B6 (control) 9 0 11.1 11.1 1.00 0.05 - 18.9 1.00 MAb-B38 24 45.8 12.5 58.3 11.2 1.20 - 104 0.02 B38+BH-103 27 7.69 0 3.70 0.03 0.003 - 0.28 < .001 MAb-REGN10987 24 16.7 45.8 62.5 13.3 1.42 - 125 0.02 REGN10987+BH-103 20 5.00 0 5.00 0.03 0.004 - 0.28 < .001 (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.13.452194; this version posted August 18, 2021. The copyright holder for this preprint 6 for histology evaluation. Like previous results, acute lung inflammation was observed with the HE stained tissue sections from the mouse pups born to the dames injected with anti-COVID-19 S1, anti-SARS S, REGN10987, and B38 (Figure 2). The lung lesions included pulmonary congestion, alveolar epithelial hyperplasia and thickening, hemorrhage, alveolar atresia, alveolar dilatation, and alveolar fusion (Figure 2A). Infiltration of inflammatory cells at the local lesion areas were also observed. In addition, inflammatory reactions such as acute kidney tubular injury, cerebral and myocardial inflammation and hemorrhage were also observed with the tissues of kidneys, brains, and hearts from the mouse pups born to the dames with the injection of the four pathogenic antibodies (Figure 3). However, the treatment of BH-103 (15 mg/kg), either the day before or at the same time as the antibody injection of the four pathogenic antibodies, significantly decreased the severity of inflammation and injuries of lungs, kidneys, brains, and hearts, compared to the mouse pups delivered to the dames injected with either of the Vehicle Rabbit-IgG Anti-COVID-19 S1 Anti- S1 + BH-103 Anti-SARS S Anti-SARS S + BH-103 Human-IgG Control-MAb MAb-REGN10987 REGN10987+ BH-103 MAb-B38 B38 + BH-103 Regn10987 Regn10987 + BH-103 B38 B38 + BH-103 B38 B38 + BH-103 Kidney Kidney Brain Brain Heart Heart A Figure 3. A: The representative images of the histological changes of lungs (top 2 rows), kidneys, brains, and hearts (bottom row) from the newborn mouse pups born to the dames injected with pathogenic anti-coronavirus antibodies of anti-Covid-19 S1, anti-SARS S, and human monoclonal anti-COVID-19 S1 antibodies (MAb) REGN-10987 and B38, and control antibodies of human IgG, rabbit IgG and CR3022-b6 (Control MAb); or the dames treated with BH-103 and antibody. B: The cytokine levels of MCP-1 in mouse sera from the newborn mouse pups born to the dames with antibody injection alone or the dames treated with BH-103 and antibody. B 0 200 400 600 800 1000 MCP-1 (pg/ml) 0 200 400 600 800 1000 MCP-1 (pg/ml) (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.13.452194; this version posted August 18, 2021. The copyright holder for this preprint 7 pathogenic antibody alone (FIG 12A). There were insignificant or minor histological changes with the lungs, kidneys, brains, and hearts from the pups born to the dames injected with the control antibodies of CR3022-b6, and the human or rabbit IgGs (Figure 3). Taken together, the in vivo data indicated that BH-103 is effective for the prevention and treatment of the inflammation and injuries of multiple organs caused by pathogenic antibodies of the COVID-19 and SARS-CoV viruses. BH-103 significantly reduced cytokine production The sera from newborn pups as mentioned above were tested for inflammatory cytokines of MCP-1, TNF-, IL-4, IL-6 and IL-10 using a 5-plex multiplex Luminex assay kit (Millipore) according to manufacturer’s instruction. The results are summarized in FIG 3B. The antibodies of anti-COVID-19 S1 and REGN10987 induced significantly higher levels of MCP-1 (Figure 3B). Nevertheless, the treatment of BH-103 significantly reduced the cytokine levels of MCP-1 (P < 0.001), compared to the treatment with the pathogenic antibody alone. The results were consistent with the results of the sickness and death rates (Table 1) and the histology changes (Figure 2). The data further indicated that BH-103 is effective in preventing or treating cytokine storms caused by pathogenic antibodies. The levels of other cytokines were not significantly elevated, probably due to the undeveloped immunity of the newborn mouse pups. Discussion The novel mechanism of action (MOA) MOA-1: Repairing and blocking antibody binding. Beside their major function as biological masks, sialic acids also act as viral receptors 8 . Certain viruses, including coronaviruses, contain receptor-destroying enzymes (RDE) that cleave a terminal sialic acid from the cellular surface9 . During an infection of a virus with RDE, the terminal sialic acids on cell surfaces can be removed, and the cellular structures become “non-self”8 (Figure 4A). Our recent study has shown that damaged lung epithelium cells with missing sialic acid are particularly vulnerable to pathogenic antibodies of the COVID-19 virus6 . Thus, repairment of the missing sialic acid can convert the sick cells (non-self) back to normal (self) and stop the subsequent autoimmune reactions (ADAA) caused by pathogenic antibodies. These autoimmune reactions are not minor side effects NANA Galactose GlcNAc Mannose Self Non-self Viral ligand (e.g. influenza virus or coronavirus) RDE Sialidase A Virus NANA-Me NANA MOA-1： Repairing and blocking Abs binding MOA-2：Blocking viral entry Immune cells not activated Loss of sialic acid, binding of pathogenic antibody, & immune activation Self Non-self B Figure 4. Mechanisms of action (MOA) of N-acetylneuraminic acid methyl ester (NANA-Me). A: loss of sialic acid makes self-cells became non-self. B. NANA-Me can easily enter cells and participate in the process of sialic acid repairment, and stop ADAA reaction by blocking binding of pathogenic antibody to self-cells (MOA-1); and NANA-Me can block viral entry by modifying a viral receptor and dramatically reducing viral binding affinity (MOA-2). (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.13.452194; this version posted August 18, 2021. The copyright holder for this preprint 8 of the infection, but rather can be the main contributors to the severity of an infection and can lead to serious clinical conditions including ARDS, cytokine storms, and even death. So far, there have been no reports that uses analogs or derivatives of sialic acid as repairers or modifiers of viral receptors on host cells, probably because most anti-viral products have focused on targeting viruses. Here, we explore a new application of an analog of N-acetylneuraminic acid (NANA), N-acetylneuraminic acid methyl ester (NANA-Me), for the prevention and treatment of serious symptoms of COVID-19 infection, through a unique MOA that is being reported for the first time. The structure of NANA-Me is close to the structure of NANA. Unlike NANA, NANA-Me can easily penetrate the cell membrane, enter cells, and participate in the sugar chain synthesis process. Therefore, NANA-Me is a good candidate for the repairment of missing sialic acid on the damaged lung epithelium cells or other cells such as inflammatory cells (Figure 4B, MOA-1). The structure recovery of the damaged cells can reduce the binding of pathogenic antibodies and stop the ADAA reactions of immune system against the damaged or inflammatory cells. These MOA of NANA-Me is supported by the in vitro and in vivo data of the current study. The in vitro data showed that NANA-Me can enhance or repair NANA expression on lung epithelium A549 cells (Figure 1). The in vivo results showed that BH-103 effectively prevented or treated the adverse reactions of pathogenic anti-spike antibodies of COVID-19 and SARS-CoV viruses (Table 1). Because the anti-spike pathogenic antibodies are also inducible by COVID-19 vaccines, BH-103 can be effective for preventing and treating the adverse reactions of COVID-19 vaccines. MOA-2: Receptor modification. Many microbes bind to mammalian tissues by recognizing specific saccharide ligands. For example, sialic acid moieties on glycoproteins are critical receptor determinants, and are related to viral attachment of influenza viruses or coronaviruses (Stevens et al., 2006, Huang et al., 2015). Therefore, saccharides and saccharide mimetics can be used to block the initial attachment of microbes to cell surfaces or block their release, thus preventing and suppressing infection (anti-adhesive). Examples of such applications include milk oligosaccharides that are believed to be natural antagonists of gastrointestinal infection in infants, and polymers that will block the binding of viruses 11 . In addition, chemical modification of sialic acids can strongly influence their properties, in particular ligand functions11. The hydroxyl groups present in both monosaccharides and oligosaccharides can be chemically modified without affecting the glycosidic linkages 11 . Natural products containing partially methylated glycans are known and a number of methyltransferases have been identified 11 . For example, O-methylation can hinder or even prevent hydrolysis of the glycosidic bond by sialidase11 . Nevertheless, most derivatives of sialic acid, including some methyl esters of NANA, are widely used for competitive inhibitors of neuraminidase of bacteria and viruses (e.g. influenza viruses) 12 . The most well-known such example is Tamiflu. NANA-Me can act as a donor of methyl organ. The replacement of NANA by NANA-Me could cause a structural or chemical modification of the viral receptor that can significantly decreased the binding affinity of the related viruses including the SARS-CoV-2 (MOA-2, Figure 4B) (Figure 2). Why in combination with NANA? One weakness of NANA-Me is that it is unstable and easily hydrolyzed. This weakness makes it hard for NANA-Me to be a good therapeutic product. Our study found that the stability of NANA-Me was improved when it was in a composition comprising NANA and under a condition of pH 4.0-5.5 (unpublished data, patent pending). Therefore, we designed the formulation of BH-103 consisted of NANA-Me and NANA with a ratio of NANA-Me:NANA at 2:1 (pH 4.43). Our in vitro and in vivo data showed that BH-103 has overcome this shortcoming and significantly improved the efficacy of NANA-Me. Thus BH-103 can be a good therapeutic medicine for the disorders caused by pathogenic antibodies of viral infections such as COVID-19. Advantages of drugs targeting viral receptors The results of this study indicate that NANA-Me (or BH-103) can chemically modify the sialic acid of the SARS-CoV-2 viral receptor and block viral entry into host cells (MOA-2, Figure 2 and Figure 4B). Based on (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.13.452194; this version posted August 18, 2021. The copyright holder for this preprint 9 the MOA-2, N-acetylneuraminic acid methyl ester or BH-103 has the potential to prevent the COVID-19 infection by blocking viral entry into host cells, and treat the infection by blocking viral spread into new cells. Future prospective in vivo studies are needed to confirm this function of NANA-Me. Given the similarities in receptor usage, sialic acid is a receptor component for not only coronaviruses, but also other viruses such as influenza viruses. The receptor modification and entry blockage by NANA-Me should be widely effective for the prevention and treatment of other infections caused by other viruses using sialic acids as receptors, particular a highly pathogenic influenza infection. As supporting evidence, our previous studies showed that formulations consisting of NANA-Me and NANA were effective for prevention and treatment of influenza infections 13 . There are a couple of advantages with therapeutics targeting viral receptors: 1) broad spectrum of efficacy. Unlike the multiple and complicated viral strains, viral receptors on host cells are limited. Thus, a therapeutic capable of modifying receptors can be effective to all the viruses and viral strains sharing the receptor. 2) anti-mutation. Effective modification of a viral receptor can block the attachment of all viruses using that receptor, regardless their strains (including mutated variants). Highlights of BH-103 Compared to other anti-viral drugs which target virus, BH-103 has the advantages below: Firstly, BH-103 acts through a unique MOA of interrupting ADAA caused by pathogenic antibodies from all viruses capable of inducing such antibodies, for example highly pathogenic coronaviruses such as COVID-19 virus and influenza viruses. Secondly, BH-103 is broadly effective for the ADAA-based diseases caused by pathogenic antibodies. Those diseases include infectious diseases, complications and sequela of infections including COVID-19 long haulers, cytokine storm and cytokine release syndrome (CRS), adverse reactions of vaccines, infection-relating autoimmune diseases, and other possible disorders inducible by pathogenic antibodies. The ADAA-based diseases further include abortion, postpartum labor, still birth and neonatal death of pregnant females, caused by an infection or by a vaccine. Thirdly, BH-103 has an excellent safety profile. A 7-day multiple dosing toxicity study with rats and dogs showed that no signs of toxicity was observed with BH-103 at the highest dose of 750 mg/kg (unpublished data). Fourthly, no drug resistance. BH-103 is resistant to viral mutations since it targets a host receptor instead of a virus. Fifthly, BH-103 is easy to produce and large amounts of it can be produced in a short amount of time. This will be helpful in fulfilling the large increase in demand for effective therapeutics experienced during a pandemic, such as the current COVID-19 pandemic. In summary, our recent study revealed “pathogenic antibodies” in viral infections. These pathogenic antibodies act through a novel mechanism of action that we have termed “Antibody Dependent Auto-Attack” (ADAA). The current study reports a formulation (BH-103) that is designed to interrupt the ADAA reactions through blocking pathogenic antibody binding. Therefore, BH-103 can be broadly effective for the diseases caused by pathogenic antibodies, such as serious COVID-19 conditions including ARDS, cytokine storms, and death. Further, BH-103 can be effective for infection-related autoimmune diseases, including those experienced by COVID-19 long haulers. Importantly, BH-103 can prevent and treat the adverse reactions of vaccines including COVID-19 vaccines. This will be particularly helpful to promote the global COVID-19 vaccination and to control the pandemic as soon as possible. Therefore, BH-103 will provide an effective resolution for the urgent clinic unmet of the COVID-19 pandemic and the other possible pandemic emerging in future. Methods Compounds Compounds of N-acetylneuraminic acid (NANA) and N-acetylneuraminic acid methyl ester (NANA-Me) were purchased (Jun Kang Biotech, Guangzhou). NANA-Me comprises the formula of C12H21NO9, and the molecular structure of: (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.13.452194; this version posted August 18, 2021. The copyright holder for this preprint 10 COOMe The compound purity was 97% for NANA, and 98% for NANA-Me. A formulation consisted of 0.5 gram of NANA-Me, 0.25 gram of NANA, and 0.175 gram of sodium citrate was made and named BH-103. A solution of BH-103 at 10 mg/ml (pH 4.43) was made, aliquoted and stored at -80℃. Freshly thawed BH-103 solution (pH 4.43) was used for each experiment. Production of human anti-SARC-CoV-2 spike monoclonal antibodies Previously reported naturally occurring human monoclonal antibodies of B38 14 , REGN10987 15 , and CR3022-B6 16 , specific to the receptor binding domain (RBD) of the spike protein one (S1) of the SARS-CoV-2 virus were re-produced as previously reported (HuaAn McAb Biotechnology). Removal of endotoxin from antibody solutions was performed as previously reported. Other antibodies The rabbit polyclonal antibodies specific for the recombinant spike one (S1) proteins of the SARS-CoV-2 virus, the recombinant spike proteins of SARS-CoV virus, were purchased from Bioss Antibodies (Beijing). S-RBD of SARS-CoV-2 The recombinant protein of the receptor binding domain (RBD) of the spike protein (S-RBD) of the SARS-CoV-2 virus (with human Fc as a tag for detection) was purchased from Sino Biological (Beijing). Digestion of A549 and NB4 cells with neuraminidase The cells of human lung epithelium cell line A549 (ATCC) , and human acute promyelocytic leukemia cell line NB4 (ATCC) were washed once with the digestion buffer for neuraminidase (Roche), the cells were centrifuged at 2000 rpm for 5 minutes, and the supernatant was discarded. The digestion buffer was consisted of 50 mM of sodium acetate, 3% BSA, and 2 mM of CaCl2 in PBS (pH5.5-6.0). The cells were resuspended with the digestion buffer and divided to multiple tubes, each tube contained about 106 of cells in 200 ml of the digestion buffer containing 50 mU of neuraminidase (Roche). The tubes were incubated at 37°C for one hour. Then the cells were washed with PBS once and proceeded for flow cytometry assay. A549 or NB4 cells without the treatment of neuraminidase were used as controls. Binding of S-RBD to NB4 cells and flow cytometry NB4 cells with or without the treatment of neuraminidase or BH-103 were tested for the binding of the S-RBD of the SARS-CoV-2 virus as mentioned above. Each 2x105 of cells in 200 ml of 1%BSA-PBS were incubated in ice with the S-RBD at a concentration of 5 mg/ml for one hour, washed once with PBS. The cells were resuspended with 200 ml of 1%BSA-PBS and 0.25 mg/ml of PE-labeled goat anti-human IgG (Abcam), incubated in ice for 30 minutes, and washed once with PBS. The cells were resuspended with 200 ml of PBS and detected with a flow cytometer of Accuri 6 (BD Biosciences). The fluorescent labeled wheat germ agglutinin (WGA), which specifically binds to sialic acid (Vector), was used as a secondary reagent for determine the sialic acid level of cells as needed. A timed-pregnant mouse model A timed-pregnant mouse model without viral infection was developed using the anti-coronavirus antibodies as previously reported. The animal experiments were performed at the Center of Laboratory Animals of Hangzhou Normal University. The protocol for the animal experiment was approved by the Laboratory Animal Welfare and Ethics Committee of Hangzhou Normal University. The CALAS No. is 2020244. The animal experiments were performed three times. Animal SPF-grade C57BL/6J pregnant mice at pregnancy (embryonic) day E13-E14 were purchased from Shanghai SLAC Laboratory Animal Co., Ltd. The animals were randomly divided into groups as needed, two pregnant mice for each group at every experiment. Antibody injection The purified and endotoxin-free IgG of the anti-coronavirus antibodies used for the animal model include rabbit polyclonal anti-COVID-19 S1, anti-SARS-CoV S, and the human monoclonal anti-COVID-19 S1, B3814, REGN1098715 , and CR3022-B6 16 . Two dosages of each antibody IgG were (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.13.452194; this version posted August 18, 2021. The copyright holder for this preprint 11 injected intraperitoneally (IP) into timed-pregnant mice once every three days at pregnancy (embryonic) day of E15 (about 26-28 g) and E18 (about 30-32 g) respectively. For each polyclonal antibody, 50 μg (microgram) for the first dose (about 2.0 mg/kg) and 60 μg for the second dose (about 2.0 mg/kg) were administrated. For each monoclonal antibody, 40 μg for the first dose (about 1.5 mg/kg) and 50 μg for the second dose (about 1.5 mg/kg) were administrated. In addition, two dosages of BH-103 injection (IP) at either day before or the same day of each antibody injection of the 4 pathogenic antibodies (anti-COVID-19 S1, anti-SARS-CoV S, REGN10987, and B38) were administrated, respectively. The body weight of the pregnant mice was measured every day before and after the antibody injection. The mouse pups were born at about E20-E21 and the healthy status including clinical signs of the newborn mouse pups were observed and recorded. The experiment was ended at day 1-2 post birth. Sample collection At the end day of the experiment, the blood samples were collected from newborn mouse pups, incubated at 4℃ for overnight, centrifuged at 3000 rpm for 5 minutes, and the supernatant was transferred to a new tube. The isolated sera were stored at -80℃ for cytokine detection. Lungs, hearts, brains, kidneys, livers, and intestines were collected from at least 3 mouse pups, fixed in formalin for 48-72 hours, went through gradient alcohol dehydration and embedded in paraffin, and tissue sections were processed. HE and immunofluorescent staining The mouse tissue sections of lungs, hearts, brains, kidneys, livers, and intestines were dewaxed and stained with hematoxylin-eosin (HE) for histology evaluation. Acknowledgement The study was supported by funding from Hangzhou HuaAn PuChu Investment Limited Partnership. Author Contributions H.W. conducted the overall study direction including experiment design, data analysis, and manuscript writing; Q.C. and D.L. conducted the in vitro cellular analysis, IgG isolation and removing endotoxin; X.W. conducted the animal experiments, sample collection and preparation of tissue section; Y.Z. conducted the process of tissue sections and HE staining, Y.C. conducted funding acquisition, resources, and supervision; X.L. conducted antibody production, data analysis and discussion. Competing Interests H.W. is the shareholder of Huirui Biopharma, X.L. and Y.C. are shareholders of HuaAn McAb Biotechnology. There are patent applications pending related to this work. References 1. Zhou, F. et al. Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study. Lancet Lond. Engl. 395, 1054–1062 (2020). 2. Fauci, A. S., Lane, H. C. & Redfield, R. R. Covid-19 - Navigating the Uncharted. N. Engl. J. Med. 382, 1268–1269 (2020). 3. Marshall, M. The lasting misery of coronavirus long-haulers. Nature 585, 339–341 (2020). 4. Dai, L. & Gao, G. F. Viral targets for vaccines against COVID-19. Nat. Rev. Immunol. 21, 73–82 (2021). 5. Meo, S. A., Bukhari, I. A., Akram, J., Meo, A. S. & Klonoff, D. C. COVID-19 vaccines: comparison of biological, pharmacological characteristics and adverse effects of Pfizer/BioNTech and Moderna Vaccines. Eur. Rev. Med. Pharmacol. Sci. 25, 1663–1669 (2021). 6. Wang, H. et al. Pathogenic antibodies induced by spike proteins of COVID-19 and SARS-CoV viruses. https://www.researchsquare.com/article/rs-612103/ v2 (2021) doi:10.21203/rs.3.rs-612103/v2. 7. Shimabukuro, T. T. et al. Preliminary Findings of mRNA Covid-19 Vaccine Safety in Pregnant Persons. N. Engl. J. Med. 384, 2273–2282 (2021). 8. Schauer, R. & Kamerling, J. P. Exploration of the Sialic Acid World. in Advances in Carbohydrate Chemistry and Biochemistry vol. 75 1–213 (Elsevier, 2018). 9. Huang, X. et al. Human Coronavirus HKU1 Spike Protein Uses O -Acetylated Sialic Acid as an (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/2021.07.13.452194; this version posted August 18, 2021. The copyright holder for this preprint 1


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    • Post n°111

    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Empty Re: Graphene ,Magnetism, the jab and the Human Brain Project

    Post  Carol Thu Sep 30, 2021 7:50 am

    Urgent message to the recently red-pilled who took the vaccine: You can still SAVE yourself!



    https://ambassadorlove.wordpress.com/2021/06/24/summary-of-the-spike-protein-protocol-david-avocado-wolf/



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    • Post n°112

    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Empty Re: Graphene ,Magnetism, the jab and the Human Brain Project

    Post  Carol Thu Sep 30, 2021 7:53 am

    Is pine needle tea the answer to covid vaccine shedding / transmission? Learn about suramin, shikimic acid and how to make your own extracts
    Sunday, May 09, 2021 by: Mike Adams
    Tags: Blood clots, COVID, covid vaccines, covid-19, Cures, extraction, Herbs, pandemic, pine needle tea, pine needles, Plague, remedies, shikimic acid, spike proteins, star anise, suramin, vaccine shedding, vaccine transmission
    Bypass censorship by sharing this link:



    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Pine-needle-tea-covid-vaccines-suramin-shikimic-acid

    (Natural News) Disclaimer: The information presented in this article and podcast are for informational purposes only. Nothing present here is intended to diagnose or treat any disease, and there are no supplements or products offered for sale in relation to this information.
    Word is spreading that pine needle tea may offer a solution against covid vaccine “shedding” or transmission, which appears to be a phenomenon where vaccinated people are spreading harmful particles or substances to others around them. See this article from a WordPress blog site called “Ambassador Love.”

    That article states:
    There is a potential antidote to the current spike protein contagion which is called Suramin. It’s found in many forests around the world, in Pine needles. Suramin has inhibitory effects against components of the coagulation cascade and against the inappropriate replication and modification of RNA and DNA. Excessive coagulation causes blood clots, mini-clots, strokes, and unusually heavy menstrual cycles.

    Pine needle tea is one of the most potent anti-oxidants there is and it’s known to treat cancer, inflammation, stress and depression, pain and respiratory infections. Pine tea also kills parasites.

    Below, find a full podcast and video that reveals two extraction methods, both of which are simple, low-tech, low-cost methods that can be used almost anywhere.

    Fresh pine needles from appropriate trees have been used for centuries as sources of vitamin C and other phytochemicals that Native Americans used to treat respiratory infections and other ailments. Vitamin C is a known cure for scurvy, as scurvy is a disease of vitamin C deficiency. Pine needles contain many other substances that appear to reduce platelet aggregation in the blood, potentially preventing blood clots that lead to strokes, heart attacks and pulmonary embolism diagnoses. (See published science sources below.)

    Pine needles have been used by indigenous populations around the world as both food and medicine for thousands of years. Many people now believe pine needles may be able to offer protection from covid spike proteins — which are engineered bioweapons found in covid vaccines — as well as covid vaccine “shedding” particles, which also appear to be biological weapons designed to achieve global depopulation.

    Dr. Judy Mikovits asserts that globalists are well aware that pine needle tea is the answer to covid depopulation weapons, and they are secretly using pine needle tea to protect themselves from the very plague they have unleashed upon the world, Mikovitz explains.



    Digging into the science behind pine needles and covid


    As a published laboratory scientist, I decided to dig into this question with the help of my laboratory knowledge and experience. Boiling fresh pine needles in order to make a tea is an extraction method that’s commonly used in food science as well as Traditional Chinese Medicine (TCM).
    Water acts as a solvent, and through heat and time, some phytochemicals in the pine needles are extracted into the water, making a pine needle tea. (This is how all tea is made.)

    As I poured over the published science research on this topic, I had two primary questions:

    1) What molecules are found in pine needles, and what are their functions in relation to halting blood clots or protecting the unvaccinated from covid vaccine shedding?

    2) What is the best extraction method to pull these molecules out of pine needles? Is there a low-tech extraction method that almost anyone can use, without needing a laboratory?

    Through research, I found that pine needles not only contain suramin, a large molecule that’s touted for various medicinal effects, but also shikimic acid.



    Shikimic acid is the basis for Tamiflu, and it’s the molecule found in Chinese Medicine herb Star Anise, that cures plagues


    Imagine my surprise when I discovered that pine needles contain shikimic acid, the same molecule found in Star Anise herb used in Traditional Chinese Medicine to treat plagues and respiratory illness.

    The Boston Herald published a story in 2010 that revealed researchers were studying extraction techniques to harvest shikimic acid from pine needles in order to provide this raw material to the pharmaceutical industry to manufacture anti-viral, anti-flu, anti-pandemic prescription medicines. From that story:

    Researchers at the University of Maine at Orono say they’ve found a new and relatively easy way to extract shikimic acid — a key ingredient in the drug Tamiflu — from pine tree needles.

    Shikimic acid can be removed from the needles of white pine, red pine and other conifer trees simply by boiling the needles in water, said chemistry professor Ray Fort Jr.

    But the extracted acid could be valuable because Tamiflu is the world’s most widely used antiviral drug for treating swine flu, bird flu and seasonal influenza. The major source of shikimic acid now is the star anise, an unusual star-shaped fruit that grows on small trees native to China.
    The research has been funded from a variety of sources, including the Maine Technology Institute, the U.S. Department of Agriculture, the National Science Foundation and the university’s chemistry department.

    One study published in ResearchGate confirms that shikimic acid offers antiplatelet-aggregating activity, meaning it helps halt blood clots:


     Content Analysis of Shikimic Acid in the Masson Pine Needles and Antiplatelet-aggregating Activity.

    From the study:
    Shikimic acid, when separated by HPLC, exhibited a dose-dependent inhibitory effect on platelet aggregation induced by adenosine diphosphate and collagen in rabbits. Because of the relative high content and good antiplatelet-aggregating activity of shikimic acid, the Masson pine needles can be used as a potential source of shikimic acid.

    …achieved about a 6% yield of shikimic acid from Masson pine needles, which is possibly the highest extracted yield from any pine species till now (Chen et al. 2014). Since pine needles are inexpensive and readily available in North Asia, North America, and Europe, there is a strong possibility to utilize them as a drug manufacturer against less available star anise species
    That study found that pine needles provide about two-thirds the shikimic acid of star anise herb:

    Masson pine needles = 5.71% shikimic acid
    Star anise = 8.95% shikimic acid

    So we know that pine needles, which are extremely common across North America, China and Europe, provide shikimic acid, a kind of “miracle” molecule that may prove incredibly useful for halting blood clots and defending people from respiratory infections.

    Further research led me to a study that used neural networking research to optimize the extraction conditions in order to carry out a highly efficient extract from pine needles: 17 Optimization of Extraction Conditions of Shikimic Acid in Pine Needles Based on Artificial Neural Network.

    That study offers the following recipe for extraction optimization:




    • Use roughly 75% alcohol (such as vodka) and 25% water

    • Use an ultrasonic cleaning machine with a stainless steel vessel

    • Set the temperature to 65 degrees C.

    • Use 280 mL of extraction solution for every 10 grams of pine needles

    • Use a duration of 25 minutes for the ultrasonic extraction




    This finished “tea” should be filtered through a coffee filter or other paper filter in order to remove large particles. The resulting liquid will contain shikimic acid, suramin, pigments and various terpenes, and will typically show some coloration and have a rather pungent taste.
    Watch this video to see how I used an ultrasonic cleaning machine to create a water extract of rosemary herb:


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    • Post n°113

    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Empty Re: Graphene ,Magnetism, the jab and the Human Brain Project

    Post  Carol Thu Sep 30, 2021 8:02 am

    Summary of the Spike Protein Protocol – David Avocado Wolf

    By David Avocado Wolf
    David Wolf’s Shop, order here: http://bit.ly/SpikeProtein
    Updated August 24th 2021:


    This is the updated Nutrition protocol to protect those who’ve been injected with spike protein, graphene oxide and mRNA and the same protocol is useful to protect those concerned with the spike protein and graphene oxide shedding (transmission) coming off those who’ve been injected. We now have evidence of the latest injections containing: mRNA, spike protein, graphene oxide, SM-102, and numerous other potentially toxic substances (also: some—but not all—injections, appear to be higher in graphene oxide and some appear to be saline placebos).


    If you know someone who has been injected and requires help, please provide them with this Nutrition Protocol:


    Most Important Elements of the Protocol (Shortlist Summary)


    Spike Protein: Shikimate neutralizes the Spike Protein


    This is the Nutrition Protocol to Prevent Damage from Spike Protein and Derivatives due to Injection and to Protect from Spike Protein Shedding (transmission):


    Shikimate Main Sources to Detox Spike Protein


    • Pine Needle Tea for Shikimic Acid or Shikimate (from green edible pine needles) There are toxic pine needles, be careful! When drinking pine needle tea, drink the oil/resin that accumulates too!
    And/Or


    • Fennel and/or Star Anise Tea
    And/Or


    • Schizandra Berry Tea


    • Iodine* (dosage depends on brand, more is not better). Iodine is a product you have to start with small dosages and build up over time.


    • Vitamin D3* (10,000 IU’s per day)


    Graphene Oxide Detox:


    • C60 (1-3 droppers full per day): One of the issues we are seeing with those who have been injected is disturbances in their energetic field (magnetism) and hot spots of inflammation. C60 is a rich-source of electrons and acts like a fire extinguisher to inflammation and simultaneously (because it bio-distributes throughout the body) drives a normalization of electron flow throughout the body. In this category, we offer two products, the traditional C60 product* is made by yours truly and the C60 Super Concentrate* is made by a carbon scientist friend of mine and contains a higher concentration of electrons. C60 is recommended to neutralize spike protein, detoxify graphene oxide and SM-102.


    • Kohlbitr Activated Charcoal: Take between 400-2000mg (1-5 capsules) a day with water.


    • NAC: N-Acetyl Cystiene is the best precursor to glutathione in the body which has the best research for neutralizing graphene oxide. Take 900-1800mg a day. Get it while you can. The US Federal communist government is trying to make NAC illegal unless you have a doctor’s prescription.


    • Enzymes (especially those containing serrapeptase and nattokinase such as VeganZyme— dosage for VeganZymes is 3 caps, twice daily):


    Serrapeptase: Serrapeptase provides the anti-inflammatory breakdown of excess and unusual protein. Dosage: 100-200 mg on an empty stomach per day.


    Nattokinase: Nattokinase has a long history of being used to prevent blood clots. 2000-4000 Fibrinolytic Units per day (2-4 capsules) with or without food.


    Special Note: Ivermectin is showing great promise against hydrogels containing graphene oxide and found on PCR test swabs, but it is a pharmaceutical, so I do not include it.


    Here is the Complete Protocol


    •Coated Silver (1-6 drops per day, depending on degree of exposure) (Coated silver blocks the sulfur-bearing protein on the spikes from entering the cell. Sulfur-rich amino acids on the spike protein interact with silver causing them to fold incorrectly).


    • NAC (N-acetyl cysteine) (accelerates detoxification and is considered a producer of the super detoxifier glutathione in the body) Dosage: 1200-2400 mg per day on an empty stomach. NAC is recommended to detoxify graphene oxide and SM-102. NAC is tough to find after the FDA recently made it illegal to purchase over the counter in the USA. Request NAC from your doctor!


    • Zinc (30-80mg per day depending on immunological pressure)


    • Vitamin D3* (10,000 IU’s per day)


    • Lyposomal Vitamin C (30ml, twice daily)*


    • Quercetin (500-1000 mg, twice daily)*


    • Iodine* (dosage depends on brand, more is not better). Iodine is a product you have to start with small dosages and build up over time.


    • PQQ* (20-40 mg per day)


    Additional recommendations:


    • Fennel and/or Star Anise Tea: These are also an excellent source of shikimate or shikimic acid (which is known to neutralize the spike protein)


    Hesperidin sources to help disable spike protein:


    • Citrus fruit (especially blood oranges, due to their high hesperidin content — hesperidin is a chalcone like quercetin that deactivates spike protein)


    • Peppermint (very high in hesperidin)


    Superherbs to help disable spike protein:


    • Triphala formulations: In Sanskrit, the word Triphala means “three fruits”: a combination of Indian gooseberry (Emblica officinalis), black myrobalan (Terminalia chebula) and belleric myrobalan (Terminalia belerica). The terminalia fruits are rich in shikimate.


    • St. John’s Wort (shikimate is found throughout the entire plant and in the flowers)


    • Comfrey Leaf (rich in shikimate)


    • Feverfew (leaves and flowers are rich in shikimate)


    • Gingko Biloba Leaf (rich in shikimate)


    • GiantHyssop or Horsemint (Agastache urtifolia) (rich in shikimate)


    • LiquidAmbar (Sweet Gum tree) A tea of the spiky seed pods is rich in shikimate.


    •Glycyrrhiza glabra (Chinese medicine’s licorice root): Glycyrrhizic acid is extracted from the root of the licorice plant inhibits spike protein.


    David Wolf’s Shop, order here: http://bit.ly/SpikeProtein
    Follow David Avocado Wolf’s channel on Telegram, here.
    ————————————————————————————
    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Photo_2021-06-12_23-28-05Scots Pine, Finland
    Order Dr. Love’s pine needle tea from Finland, here.
    Follow Dr. Ariyana Love’s channel on Telegram, here.
    Please see: PINE TEA: Possible Antidote for Spike Protein Transmission


    Editors Note:
    David Avocado Wolf’s products are all from natural, organic sources. C60 and Iodine when consumed in a synthetic chemical form are poisons, so please be aware of this.


    For example, Iodine added to table salt is poison to the body. However, Iodine can be easily absorbed from vegetables.


    I recommend replacing table salt with Himalayan salt which has 87 minerals which are the same saline as your blood.
    Tagged David Avocado Wolf, Dr. Ariyana Love, Dr. Love, Fennel Star Anise, Finland, pine needle tea, protocol, shedding, Shikimate, spike protein, Telegram, transmission, vaccine, Vitamin C, Vitamin D


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    What is life?
    It is the flash of a firefly in the night, the breath of a buffalo in the wintertime. It is the little shadow which runs across the grass and loses itself in the sunset.
    With deepest respect ~ Aloha & Mahalo, Carol
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    • Post n°114

    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Empty Re: Graphene ,Magnetism, the jab and the Human Brain Project

    Post  Carol Thu Sep 30, 2021 8:27 am

    Initial Gene Study Showed ASEA REDOX Affected Important Signaling Pathway Genes



    https://mediafilelibrary.myasealive.com/src/media/xmfl/file/ASEA%20REDOX%20Gene%20Science%20Summary%20EN.pdf



    Dr. Ariyana Love
    https://drariyanalove.myasealive.com/



    https://wellevate.me/rima-laibow



    NAC vs NAD vs NR vs Niacin vs NMN: What Are the Differences?

    September 08, 2021
    NAD is derived from Nicotinamide Riboside (NR). NR is one of the 3 main forms of Niacin (vitamin B3).

    https://onedaymd.aestheticsadvisor.com/2020/09/nac-nr-nad.html


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    It is the flash of a firefly in the night, the breath of a buffalo in the wintertime. It is the little shadow which runs across the grass and loses itself in the sunset.
    With deepest respect ~ Aloha & Mahalo, Carol
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    • Post n°115

    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Empty Re: Graphene ,Magnetism, the jab and the Human Brain Project

    Post  Carol Mon Oct 04, 2021 7:50 pm

    This is scary stuff.

    More people around the world are looking at blood samples from vaxxed people.
    Stacked red blood cells, foreign materials, metals, etc.

    https://www.bitchute.com/video/F6GzY383zklU/

    https://www.bitchute.com/video/F6GzY383zklU/


    _________________
    What is life?
    It is the flash of a firefly in the night, the breath of a buffalo in the wintertime. It is the little shadow which runs across the grass and loses itself in the sunset.
    With deepest respect ~ Aloha & Mahalo, Carol

    mudra likes this post

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    • Post n°116

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    Post  mudra Wed Oct 06, 2021 7:27 am

    Main stream Science article:

    Role of nano materials in diagnosis, prevention and therapy of Covid19

    Section 2.1 is devoted to graphene and graphene oxyde

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146202/#!po=5.82822
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    • Post n°117

    Graphene ,Magnetism, the jab and the Human Brain Project - Page 5 Empty Re: Graphene ,Magnetism, the jab and the Human Brain Project

    Post  Carol Mon Oct 18, 2021 9:27 am

    Graphene Oxide DETOX

    9/21/21. Summary of the Spike Protein and Graphene Oxide Detoxification Protocol

    http://bit.ly/SpikeProtein

    This is the updated Nutrition protocol to protect those who’ve been injected with spike protein, graphene oxide and mRNA and the same protocol is useful to protect those concerned with the spike protein and graphene oxide shedding coming off those who’ve been injected. We now have evidence of the latest injections containing: mRNA, spike protein, graphene oxide, SM-102, and numerous other potentially toxic substances (also: some—but not all—injections, appear to be higher in graphene oxide and some appear to be saline placebos).

    If you know someone who has been injected and requires help, please provide them with this Nutrition Protocol:

    Most Important Elements of the Protocol (Shortlist Summary)

    Spike Protein: Shikimate neutralizes the Spike Protein

    Shikimate Main Sources to Detox S.pike Protein

    • Pine Needle Tea for shikimic acid or shikimate (from green edible pine needles) There are toxic pine needles, be careful! When drinking pine needle tea, drink the oil/resin that accumulates too!
    And/Or

    • Fennel and/or Star Anise Tea
    And/Or

    • Schizandra Berry Tea

    • Iodine* (dosage depends on brand, more is not better). Iodine is a product you have to start with small dosages and build up over time.

    • Vitamin D3* (10,000 IU’s per day)

    Graphene Oxide Detox:

    • C60 (1-3 droppersfull per day):     One of the issues we are seeing with those who have been injected is disturbances in their energetic field (magnetism) and hot spots of inflammation. C60 is a rich-source of electrons and acts like a fire extinguisher to inflammation and simultaneously (because it bio-distributes throughout the body) drives a normalization of electron flow throughout the body. In this category, we offer two products, the traditional C60 product is made by yours truly and the C60 SuperConcentrate is made by a carbon scientist friend of mine and contains a higher concentration of electrons. C60 is recommended to neutralize s.pike protein, detoxify graphene oxide and SM-102.

    • Kohlbitr Activated Charcoal: Take between 400-2000mg (1-5 capsules) a day with water.

    • NAC: N-Acetyl Cystiene is the best precursor to glutathione in the body which has the best research for neutralizing graphene oxide. Take 900-1800 mg a day. Get it while you can. The US Federal communist government is trying to make NAC illegal unless you have a doctor’s prescription.

    • Enzymes (especially those containing serrapeptase and nattokinase such as VeganZyme— dosage for VeganZymes is 3 caps, twice daily):
    Serrapeptase: Serrapeptase provides the anti-inflammatory breakdown of excess and unusual protein. Dosage: 100-200 mg on an empty stomach per day.
    Nattokinase: Nattokinase has a long history of being used to prevent blood clots. 2000-4000 Fibrinolytic Units per day (2-4 capsules) with or without food.

    Special Note: Ivermectin is showing great promise against hydrogels containing graphene oxide and found on PCR test swabs, but it is a pharmaceutical, so I do not include it.

    Here is the Complete Protocol

    •Coated Silver (1-6 drops per day, depending on degree of exposure) (Coated silver blocks the sulfur-bearing protein on the spikes from entering the cell. Sulfur-rich amino acids on the spike protein interact with silver causing them to fold incorrectly).

    • NAC (N-acetyl cysteine) (accelerates detoxification and is considered a producer of the super detoxifier glutathione in the body) Dosage: 1200-2400 mg per day on an empty stomach. NAC is recommended to detoxify graphene oxide and SM-102. NAC is tough to find after the FDA recently made it illegal to purchase over the counter in the USA. Request NAC from your doctor!

    • Zinc (30-80mg per day depending on immunological pressure)
    • Vitamin D3* (10,000 IU’s per day)
    • Lyposomal Vitamin C (30ml, twice daily)*
    • Quercetin (500-1000 mg, twice daily)*
    • Iodine* (dosage depends on brand, more is not better). Iodine is a product you have to start with small dosages and build up over time.
    • PQQ* (20-40 mg per day)

    Shikimate Main Sources:
    • Pine Needle Tea for shikimic acid or shikimate (from green edible pine needles)     There are toxic pine needles, be careful! When drinking pine needle tea, drink the oil/resin that accumulates too! Shikimate, shikimic acid and their derivatives possess: cancer fighting, antiviral, antimicrobial, anticoagulant and antithrombotic properties.
    • Fennel and/or Star Anise Tea: These are also an excellent source of shikimate or shikimic acid (which is known to neutralize the spike protein)
    • Schizandra Tea: Ranked in the top three of all superherbs on Earth. Schizandra berries are rich in shikimate amongst numerous extraordinary other properties.

    • C60 (1-3 droppersfull per day): One of the issues we are seeing with those who have been injected is disturbances in their energetic field (magnetism) and hot spots of inflammation. C60 is a rich-source of electrons and acts like a fire extinguisher to inflammation and simultaneously (because it bio-distributes throughout the body) drives a normalization of electron flow throughout the body. In this category, we offer two products, the traditional C60 product is made by yours truly and the C60 SuperConcentrate is made by a carbon scientist friend of mine and contains a higher concentration of electrons. C60 is recommended to neutralize s.pike protein, detoxify graphene oxide and SM-102.

    • Charcoal (2-4 capsules a day): Charcoal is the pre-eminent detoxifier and when taken on an empty stomach, works its way down into the intestines and activates a blood purification process known as “interstitial dialysis”. Our Kohlbitr product is the premier activated coconut charcoal in the world and we also now offer the more gentle birch charcoal.

    Hesperidin sources to help disable s.pike protein:
    • Citrus fruit (especially blood oranges, due to their high hesperidin content — hesperidin is a chalcone like quercetin that deactivates spike protein)
    • Peppermint (very high in hesperidin)

    Superherbs to help disable s.pike protein:
    • Triphala formulations: In Sanskrit, the word Triphala means "three fruits”: a combination of Indian gooseberry (Emblica officinalis), black myrobalan (Terminalia chebula) and belleric myrobalan (Terminalia belerica). The terminalia fruits are rich in shikimate.
    • St. John’s Wort (shikimate is found throughout the entire plant and in the flowers)
    • Comfrey Leaf (rich in shikimate)
    • Feverfew (leaves and flowers are rich in shikimate)
    • Gingko Biloba Leaf (rich in shikimate)
    • GiantHyssop or Horsemint (Agastache urtifolia) (rich in shikimate)
    • LiquidAmbar (Sweet Gum tree) A tea of the spiky seed pods is rich in shikimate.
    •Glycyrrhiza glabra (Chinese medicine’s licorice root): Glycyrrhizic acid is extracted from the root of the licorice plant inhibits spike protein. https://pubmed.ncbi.nlm.nih.gov/33041173/

    Foods
    • Carrots and Carrot Juice (rich in Shikimate)
    • Dandelion Leaf (Common dandelion (Taraxacum officinale) efficiently blocks the interaction between ACE2 cell surface receptor and spike protein D614, mutants D614G, N501Y, K417N and E484K in vitro)

    Plant Sprouts
    • Wheatgrass and Wheatgrass Juice (the young blades are high in shikimate)
    • Legume family beans that are generally considered to be rich in shikimate. I have found testing of 5 sprouts and all were rich in shikimate: red kidney bean (Phaseolus vulgaris), moth bean (Vigna aconitifolia), soy bean (Glycine max), mung bean (Vigna radiata) and alfalfa (Medicago saliva). All these were analysed for their shikimic acid content during germination: so the sprouting process is key to activate shikimate production.

    Enyzmes
    •Nattokinase (enzyme) and Natto is a traditional Japanese food made from soybeans fermented with Bacillus subtilis var. natto. Natto (also rich in vascular protective Vitamin K2) and Nattokinase have a history of being used to prevent blood clots. The idea is here is that the enzyme Nattokinase goes to work dissolving clots. 2000-4000 Fibrinolytic Units per day (2-4 capsules) with or without food.

    9/15/21 Greaka’s C60 https://www.c-60.com/

    8/2021 Graphene oxide detox https://diannemarshallreport.com/graphene-oxide-detox/

    9/1/21 Biosil: https://myersdetox.com/activated-silica/

    H2O2 therapy:

    https://baywire.blogspot.com/2011/08/one-minute-cure-my-results-from.html

    https://www.theoneminutemiracleinc.com/products/organic-h2o2-hydrogen-peroxide-food-grade-certified-12-oz?variant=18857590852



    _________________
    What is life?
    It is the flash of a firefly in the night, the breath of a buffalo in the wintertime. It is the little shadow which runs across the grass and loses itself in the sunset.
    With deepest respect ~ Aloha & Mahalo, Carol
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      Current date/time is Thu May 09, 2024 11:02 pm